| No | Car | Driver | Manuf. | Sponsor | Owner |
| 1 | 1 | Jamie McMurray | Chevy | McDonald’s | Chip Ganassi Racing |
| 2 | 2 | Brad Keselowski | Ford | Alliance Truck Parts | Team Penske |
| 3 | 3 | Austin Dillon | Chevy | Dow | Richard Childress Racing |
| 4 | 4 | Kevin Harvick | Chevy | Jimmy John’s | Stewart-Haas Racing |
| 5 | 5 | Kasey Kahne | Chevy | Great Clips | Hendrick Motorsports |
| 6 | 6 | Trevor Bayne | Ford | AdvoCare | Roush Fenway Racing |
| 7 | 7 | Regan Smith | Chevy | APC | Tommy Baldwin Racing |
| 8 | 10 | Danica Patrick | Chevy | Nature’s Bakery | Stewart-Haas Racing |
| 9 | 11 | Denny Hamlin | Toyota | FedEx Freight | Joe Gibbs Racing |
| 10 | 13 | Casey Mears | Chevy | GEICO | Germain Racing |
| 11 | 14 | Ty Dillon (i) | Chevy | Bass Pro Shops / Tracker Boats |
Stewart-Haas Racing |
| 12 | 15 | Clint Bowyer | Chevy | 5-Hour Energy | Hscott Motorsports |
| 13 | 16 | Greg Biffle | Ford | Cheez-It | Roush Fenway Racing |
| 14 | 17 | Ricky Stenhouse Jr. | Ford | Fastenal | Roush Fenway Racing |
| 15 | 18 | Kyle Busch | Toyota | Skittles | Joe Gibbs Racing |
| 16 | 19 | Carl Edwards | Toyota | Stanley | Joe Gibbs Racing |
| 17 | 20 | Matt Kenseth | Toyota | Dollar General | Joe Gibbs Racing |
| 18 | 21 | Ryan Blaney | Ford | Motorcraft / Quick Lane Tire & Auto Center |
Wood Brothers Racing |
| 19 | 22 | Joey Logano | Ford | Pennzoil | Team Penske |
| 20 | 23 | David Ragan | Toyota | Dr Pepper | BK Racing |
| 21 | 24 | Chase Elliott | Chevy | NAPA Auto Parts | Hendrick Motorsports |
| 22 | 27 | Paul Menard | Chevy | Richmond / Menards | Richard Childress Racing |
| 23 | 30 | Josh Wise | Chevy | Curtis Key Plumbing | The Motorsports Group |
| 24 | 31 | Ryan Newman | Chevy | Grainger | Richard Childress Racing |
| 25 | 32 | Joey Gase (i) | Ford | TBA | GO FAS Racing |
| 26 | 34 | Chris Buescher | Ford | Dockside Logistics | Front Row Motorsports |
| 27 | 38 | Landon Cassill | Ford | Snap Fitness | Front Row Motorsports |
| 28 | 41 | Kurt Busch | Chevy | Monster Energy / Haas Automation |
Stewart-Haas Racing |
| 29 | 42 | Kyle Larson | Chevy | Target | Chip Ganassi Racing |
| 30 | 43 | Aric Almirola | Ford | Smithfield | Richard Petty Motorsports |
| 31 | 44 | Brian Scott | Ford | Albertson’s Co. / White Tail |
Richard Petty Motorsports |
| 32 | 46 | Michael Annett | Chevy | Pilot Flying J | Hscott Motorsports |
| 33 | 47 | A.J. Allmendinger | Chevy | Fry’s / Scott Products |
JTG Daugherty Racing |
| 34 | 48 | Jimmie Johnson | Chevy | Lowe’s Pro Services | Hendrick Motorsports |
| 35 | 78 | Martin Truex Jr. | Toyota | Bass Pro Shops / TRACKER Boats |
Furniture Row Racing |
| 36 | 83 | Matt Dibenedetto | Toyota | Schluter Systems | BK Racing |
| 37 | 88 | Dale Earnhardt Jr. | Chevy | Axalta | Hendrick Motorsports |
| 38 | 95 | Michael McDowell | Chevy | Thrivent Financial | Circle Sport-Leavine Family Racing |
| 39 | 98 | Cole Whitt | Chevy | TBA | Premium Motorsports |
(i) = driver not running for 2016 Sprint Cup Series Championship Drivers Points