Daytona 500 Starting Lineup
Daytona International Speedway
February 21, 2016
| Pos | Car# | Driver | Manufacturer | Team |
| 1 | 24 | Chase Elliott | Chevrolet | Hendrick Motorsports |
| 2 | 20 | Matt Kenseth | Toyota | Joe Gibbs Racing |
| 3 | 88 | Dale Earnhardt Jr. | Chevrolet | Hendrick Motorsports |
| 4 | 18 | Kyle Busch | Toyota | Joe Gibbs Racing |
| 5 | 22 | Joey Logano | Ford | Team Penske |
| 6 | 1 | Jamie McMurray | Chevrolet | Chip Ganassi Racing w/FS |
| 7 | 21 | Ryan Blaney | Ford | Wood Brothers Racing |
| 8 | 41 | Kurt Busch | Chevrolet | Stewart-Haas Racing |
| 9 | 4 | Kevin Harvick | Chevrolet | Stewart-Haas Racing |
| 10 | 19 | Carl Edwards | Toyota | Joe Gibbs Racing |
| 11 | 11 | Denny Hamlin | Toyota | Joe Gibbs Racing |
| 12 | 95 | Ty Dillon | Chevrolet | Leavine Family Racing |
| 13 | 5 | Kasey Kahne | Chevrolet | Hendrick Motorsports |
| 14 | 42 | Kyle Larson | Chevrolet | Chip Ganassi Racing w/FS |
| 15 | 16 | Greg Biffle | Ford | Roush Fenway Racing |
| 16 | 10 | Danica Patrick | Chevrolet | Stewart-Haas Racing |
| 17 | 34 | Chris Buescher | Ford | Front Row Motorsports |
| 18 | 14 | Brian Vickers | Chevrolet | Stewart-Haas Racing |
| 19 | 17 | Ricky Stenhouse Jr. | Ford | Roush Fenway Racing |
| 20 | 93 | Matt DiBenedetto | Toyota | BK Racing |
| 21 | 3 | Austin Dillon | Chevrolet | Richard Childress Racing |
| 22 | 46 | Michael Annett | Chevrolet | HScott Motorsports |
| 23 | 6 | Trevor Bayne | Ford | Roush Fenway Racing |
| 24 | 38 | Landon Cassill | Ford | Front Row Motorsports |
| 25 | 2 | Brad Keselowski | Ford | Team Penske |
| 26 | 48 | Jimmie Johnson | Chevrolet | Hendrick Motorsports |
| 27 | 7 | Regan Smith | Chevrolet | Tommy Baldwin Racing |
| 28 | 78 | Martin Truex Jr. | Toyota | Furniture Row Racing |
| 29 | 23 | David Ragan | Toyota | BK Racing |
| 30 | 47 | AJ Allmendinger | Chevrolet | JTG Daugherty Racing |
| 31 | 15 | Clint Bowyer | Chevrolet | HScott Motorsports |
| 32 | 13 | Casey Mears | Chevrolet | Germain Racing |
| 33 | 32 | Bobby Labonte | Ford | Go Green Racing |
| 34 | 43 | Aric Almirola | Ford | Richard Petty Motorsports |
| 35 | 44 | Brian Scott | Ford | Richard Petty Motorsports |
| 36 | 83 | Michael Waltrip | Toyota | BK Racing |
| 37 | 27 | Paul Menard | Chevrolet | Richard Childress Racing |
| 38 | 31 | Ryan Newman | Chevrolet | Richard Childress Racing |
| 39 | 59 | Michael McDowell | Chevrolet | Leavine Family Racing |
| 40 | 26 | Robert Richardson Jr. | Toyota | BK Racing |